Abstract
Sequence-based approaches to prokaryotic systematics and typing represent a modern and promising strategy in epidemiology and environmental microbiology. GenEnv, a database-driven system for bacterial typing, was developed in order to provide user friendly tools for supporting biomolecular analysis of bacteria. The family Vibrionaceae represents a heterogeneous
taxon of aquatic microrganisms, harbouring a plethora of genomes currently analyzed by different molecular techniques.
Under the query ?Vibrio?, GenEnv retrieved 256 organisms, included in a total number of 19 families. Overall, 548 sequences, comprising 16S rRNA(n = 402), rpoB (n = 1), gyrB (n = 145) were available. In addition, GenEnv system allowed
primer design, homology analysis and restriction maps, for immediate applications to the study of Vibrionaceae.
