PCR-Based Assay for Rapid and precise Detection of Pseudomonas aeruginosa from Other Pseudomonas Species recovered from burned Patients



Background: Pseudomonas aeruginosa is an important life threatening nosocomial pathogen which plays a prominent role in wound infections of burned patients. We designed this study to identify the isolates of P. aeruginosa recovered from burned patients at the genus and species level through primers targeting oprI and oprL genes; and analyzing their antimicrobial resistance pattern.

Methods: during a 5 months period, wound samples were taken from burned patients and plated on MacConkey agar. All suspected colonies were primarily screened for P. aeruginosa by a combination of phenotypic tests. Molecular identifications of colonies were done using specific primers for oprI and oprL genes.

Results: during a 5 months period bacterial isolates recovered from burn wound infections. Based on phonotypical identification tests, 171(34.8%) P. aeruginosa isolates, identified among burned patients; whereas by molecular techniques, just 133 p. aeruginosa yielded amplicon of oprL gene using species-specific primers, verifying the identity of P. aeruginosa; and the others yielded amplicon of oprI gene using genus-specific primers confirming the identity of fluorescent pseudomonads.

Conclusion: This study indicates that molecular detection of P. aeruginosa in burn patients employing the OprL gene target is a useful technique in the early and precise detection of P. aeruginosa. It’s considerable that for the best aggressive antibiotic recommendation of P. aeruginosa strains at earlier stage, beside phenotypic tests, PCR detection should be done. It also has significant benefits on clinical outcomes.