Water holds great relevance in various biological and biochemical systems. Water behaves as an excellent solvent, a reactant, a product and a catalyst of the reaction. The organisation of the water molecules, synergised by hydrogen bonds, builds up the structure of the water clusters. These water clusters significantly influence biological functions. To study the domain of water clusters using Ion mobility mass spectrometry with surface activated chemical ionisation. The experimental analysis was aimed to determine the water behaviour in terms of cluster formation before and after the application of a physical effect, namely low-frequency irradiation. A sanist platform-based spectrometer, manufactured by ISB srl with SACI version for protein analysis, was used as the equipment. Furthermore, for samples, we used pure de-ionised water, a part of which was used virgin, and another part was irradiated. Ion-mobility mass spectrometry (IM-MS) procedure was adopted as the experimental method. An electromagnetic frequency fields generator was used to subject the test samples to electromagnetic radiations between 7 Hz to 80 Hz. The presence of neutral water species was confirmed in the water samples. For the same m/z, water ion clusters in the untreated water were found to have a much higher intensity than the electromagnetically treated water. The presence of a water cluster near the (M+H)+ in electromagnetically treated dilute arginine solution was also confirmed. It is possible to detect water ion clusters by using Ion mobility mass spectrometry and SACI with low surface potential (47 V). The water cluster formation and its characteristics were found to be different in the treated and non-treated water. The electromagnetic radiations of low frequency seem to affect the hydrogen bonds of the water molecules.
 Ellabaan MMH, Ong YS, Nguyen QC, Kuo J-L. Evolutionary discovery of transition states in water clusters. J Theor Comput Chem 2012;11:965-95. https://doi.org/10.1142/S0219633612500654
 Szent-Györgyi A. Biology and pathology of water. Perspect Biol Med 1971;14:239-49. https://doi.org/10.1353/pbm.1971.0014
 Brini E, Fennell CJ, Fernandez-Serra M, Hribar-Lee B, Lukšič M, Dill KA. How water’s properties are encoded in its molecular structure and energies. Chem Rev 2017;117:12385-414. https://doi.org/10.1021/acs.chemrev.7b00259
 Chaplain MF. Water: its importance to life. Biochem Mol Biol Educ 2001;29:54-9. https://doi.org/10.1016/S1470-8175(01)00017-0
 Ludwig R. Water: From Clusters to the Bulk. Angew Chem Int Ed 2001;40:1808-27.
 Chaplin M. Water Structure and Science. 2021. Available at: https://water.lsbu.ac.uk/water/clusters_overview.html. Accessed on: 26/03/2022.
 Cristoni S, Rubini S, Bernardi LR. Development and applications of surface-activated chemical ionisation. Mass Spectrom Rev 2007;26:645-56. https://doi.org/10.1002/mas.20143
 Cristoni S, Bernardi LR, Biunno I, Tubaro M, Guidugli F. Surface-activated no-discharge atmospheric pressure chemical ionisation. Rapid Commun Mass Spectrom 2003;17:1973-81. https://doi.org/10.1002/rcm.1141
 Arzoni A, Bernardi LR, Cristoni S. In-source Cloud Ion mobility mass spectrometry. Rapid Commun Mass Spectrom 2015;29:690-4. https://doi.org/10.1002/rcm.7136
 Chaplin M. Water structure and Science. 2021. Available at: https://water.lsbu.ac.uk/water/magnetic_electric_effects.html. Accessed on: 25/03/2022.
 Konig S, Fales HM. Formation and decomposition of water clusters as observed in a triple quadrupole mass spectrometer. J Am Soc Mass Spectrom 1998;9:814-22. https://doi.org/10.1016/S1044-0305(98)00044-0
 Keutsch FN, Saykally RJ. Water clusters: Untangling the mysteries of the mysteries of the liquid, one molecule at a time. PNAS 2001;98:10533-40. https://doi.org/10.1073/pnas.191266498
 Silvestroni P. Fondamenti di chimica. 4th ed. Italy: CEA 1996.
 Roy R, Tiller WA, Bell I, Hoover MR. The structure of liquid water; novel insights from materials research; potential relevance to homeopathy. Mater Res Innov 2005;9:98-103. https://doi.org/10.1080/14328917.2005.11784911
 Frank HS, Wen WY. Ion-solvent interaction. Structural aspects of ion-solvent interaction in aqueous solutions: a suggested picture of water structure. Discussion Faraday Society 1957;24:133-40. http://dx.doi.org/10.1039/df9572400133
 Del Giudice E, Preparata G, Vitiello G. Water as a Free Electric Dipole Laser. Phys Rev Lett 1988;61:1085. https://doi.org/10.1103/PhysRevLett.61.1085
 Vallée P, Lafait J, Legrand L, Mentré P, Monod MO, Thomas Y. Effects of pulsed low frequency electromagnetic fields on water characterised by light scattering techniques: role of bubbles. Langmuir 2005;21:2293-9. https://doi.org/10.1021/la047916u
 Ho MW. Illuminating water and life: Emilio Del Giudice. Electromagn Biol Med 2015;34:113-22. https://doi.org/10.310 9/15368378.2015.1036079
 Preparata G. QED Coherence in Matter. 1st ed. Milano: World Scientific 1995. https://doi.org/10.1142/2738
 Germano R. Water’s quantum structures and life. Electromagn Biol Med 2015;34:133-7. https://doi.org/10.3109/15368378.20 15.1036074
 Del Giudice E, De Ninno A, Fleischmann M, Mengoli G, Milani M, Talpo G, Vitiello G. Coherent quantum electrodynamics in living matter. Electromagn Biol Med 2005;24:199-210. https://doi.org/10.1080/15368370500379574
 Pocker Y. Water in enzyme reactions: biophysical aspects of hydration-dehydration processes. Cell Mol Life Sci 2000;57:1008-17. https://doi.org/10.1007/PL00000741
 Arani R, Bono I, Del Guidice E, Preparata G. QED coherence and the thermodynamics of the water. Int J Mod Phys B 1995;9:1813-41. https://doi.org/10.1142/S0217979295000744
 Del Giudice E, Vitiello G. The role of the electromagnetic field in the formation of domains in the process of symmetry breaking phase transition. Phys Rev 2006;74:022105. https://doi.org/10.1103/PhysRevA.74.022105
 Ho MW. Living Rainbow H20. 1st ed. World Scientific Publishing Company 2012. https://doi.org/10.1142/8418
 Elia V, Ausanio G, Gentile F, Germano R, Napoli E, Niccoli M. Experimental evidence of stable water nanostructures in extremely dilute solutions, at standard pressure and temperature. Homeopathy 2014;103:44-50. https://doi.org/10.1016/j. homp.2013.08.004
 Yinnon CA, Yinnon TA. Domains in aqueous solutions: theory and experimental evidence. Mod Phys Lett A 2009;23:1959-73. https://doi.org/10.1142/S0217984909020138