Prevalence and Predictors of Risk Factors for Brucellosis Transmission by Meat Handlers and Traditional Healers’ Risk Practices in Ibadan, Nigeria


public health
risk factors


Introduction: Brucellosis is endemic in Nigeria and risk factors enhancing its transmission are prevalent.

Methods: Following serological evidence of brucellosis and isolation of B. abortus from slaughtered cattle in Ibadan, Nigeria, we administered a semi-structured questionnaire to determine the prevalence and predictors of eating and selling bovine gravid uterus among 350 meat handlers from five major meat processing facilities and conducted key informant interview for five leading traditional healers to document its use. Data were analysed using Stata 12.

Results: The prevalence of eating and selling gravid uterus were respectively, 29.7% and 40.3%. Being meat/offal processor (OR=1.9, 95%CI: 1.11-3.3, P =0.008), male (OR=1.7, 95%CI: 0.1-3.03, P =0.023) and not knowing that eating undercooked contaminated gravid uterus could expose humans to brucellosis (OR=2.4, 95%CI: 1.56-3.62, P =0.000) were strong predictors of eating gravid uterus. Similarly, being adult (OR=1.7, 95%CI: 1.08-2.57, P =0.02) and inadequate knowledge of brucellosis as a zoonosis (OR=2.9, 95%CI: 1.71-4.92, P =0.000) strongly predicted selling gravid uterus. Qualitative data from the traditional healers revealed using gravid uterus as special medicinal preparations to hasten parturition in overdue pregnancies, treat infertility and old age diseases in humans.

Discussions: We demonstrated high prevalence of risk factors for brucellosis transmission and some meat handlers’ socio-demographic characteristics including brucellosis knowledge-based markers as predictors of these factors. The traditional healers’ practices portend a challenge to the current brucellosis control strategy. These findings provide insights into designing all-inclusive health programmes aimed at controlling brucellosis spread in Nigeria and other similar settings in developing countries.


. World Health Organization, Food and Agriculture Organization of the United Nations &

World Organization for Animal Health. Report of the WHO/FAO/OIE joint

consultation on emerging zoonotic diseases, World Health Organization, Geneva 2004.

. Pappas G, Papadimitriou P, Akritidis N, et al. The new global map of human brucellosis. Lancet Infect Dis 2006;6:91-99.

Dean AS, Crump L, Greter H, et al. Global burden of human brucellosis: a systematic review of disease frequency. PLoS Negl Trop Dis 2012;6(10):e1865.

Minas M, Minas A, Gourgulianis K, et al. Epidemiological and clinical aspects of human brucellosis in Central Greece. Jpn J Infect Dis 2007;60(6):362–366.

Ciftci E, Ince E, Dog˘ru U. Pyrexia of unknown origin in children: a review of 102 patients from Turkey. Ann Trop Paediatr 2003;23:259-263.

Pathak AD, Dubal ZB, Doijad S, et al. Human brucellosis among pyrexia of unknown origin cases and occupationally exposed individuals in Goa Region, India. Emerg Hlth Threats J 2014;7:23846.

Bax HI, Van Veelen ML, Gyssen IC. Brucellosis, an uncommon and frequently delayed diagnosis. Netherlands J of Med 2007:2:352–355.

Corbel MJ, Nicoletti PL, Young EJ. Brucellosis: epidemiology and prevalence worldwide. In: Young EJ, Corbel MJ, editors. Brucellosis: Clinical and laboratory aspects. Boca Raton: CRC Press; 1989.

Swai ES, Schoonman L. Human brucellosis: seroprevalence and risk factors related to high risk occupational groups in Tanga Municipality, Tanzania. Zoonoses Public Hlth


Georgiou P, Nicholas A, Mile B, et al. Brucellosis. New Eng J Med 2005; 352:2325–

John K, Fitzpatrick J, French N, et al. Quantifying risk factors for human brucellosis in rural northern Tanzania. PLoS ONE 2010;5: e9968.

Shirima GM, Fitzpatrick J, Kunda JS, et al. The role of livestock keeping in human brucellosis trends in livestock keeping communities in Tanzania. Tanzanian J Hlth Res 2010;12(3):203-207.

Baba MM, Sarkindared SE, Brisibe F. Serological evidence of brucellosis among predisposed patients with pyrexia of unknown origin in the north eastern Nigeria. Central European J Public Hlth 2001; 3:158–161.

Ofukwu AR, Yohanna CA, Abuh HA. Brucella infection among hospital patients in Makurdi, North central Nigeria. Niger J Parasitol 2006;16:73–78.

Aworh MK, Okolocha E, Kwaga J, et al. Human brucellosis: seroprevalence and associated exposure factors among abattoir workers in Abuja, Nigeria – 2011. Pan Afr Med J 2013;16:103.

Alausa O, Awoseyi A. Brucellosis: the situation in Western Nigeria. Trop Geogr Med 1976;28(1):54-59.

Ishola OO, Ogundipe GAT. Sero-prevalence of brucellosis in trade cattle slaughtered in

Ibadan, Nigeria. Trop Vet 2001;19:17-20.

Cadmus SIB, Ijagbone IF, Oputa HE, et al. Serological survey of brucellosis in livestock animals and workers in Ibadan, Nigeria. Afr J Biomed Res 2006;9:163–168.

Cadmus SIB, Osikoya IE, Adesokan HK. Brucellosis in trade cattle in Lagos State: an Investigation of two Abattoirs. Nig Vet J 2009;29(4):43-46.

Bwala DG, McCrindle C, Fasina FO, et al. Abattoir characteristics and seroprevalence of bovine brucellosis in cattle slaughtered at Bodija Municipal Abattoir, Ibadan, Nigeria. J Vet. Med Animl Hlth 2015;7(5):164-168.

Crawford RP, Huber JD, Adams LG. Epidemiology and surveillance. In: Nielsen K, Duncan BO, eds. Animal brucellosis. CRC Press 1990, pp. 131–151.

McEntee M. The uterus: degenerative and inflammatory lesions. In: Reproductive

pathology of domestic mammals. Academic Press, Inc. San Diego, California 92101, 2012.

Momoh HA, Ijale GO, Ajogi I, et al. Risk factors and level of awareness of canine brucellosis in Jos, Plateau State, Nigeria. J Vet Med Animl Hlth 2015;7(2):39-44.

Adamu NN, Ajogi I. Serological investigations of camels (Camelus dromedarius) slaughtered at Kano municipal abattoir for evidence of brucellosis. Trop Vet 1999;18:45–48.

International Livestock Research Institute. Mapping of poverty and likely zoonoses hotspots. Nairobi, Kenya: ILRI, 2012.

(Accessed June 24, 2014).

Wahab B. Ibadan: a rapidly growing city in need of a master plan. Paper read at the Architects’ Congress/Annual General Meeting organized by the Nigerian Institute of Architects, Oyo State Chapter, held at Penrose Event Centre, Obafemi Awolowo Avenue, Old Bodija Estate, Ibadan 2011. Available at:

(Accessed August 15, 2015).

Adamu NB, Adeniyi SO, Adamu SG, et al. Seroprevalence of brucellosis among livestock workers at Maiduguri cattle market, Borno State, North Eastern, Nigeria. J. Public Hlth Epidemiol 2015;7(8):253-257.

Adesokan HK, Alabi PI, Stack JA, et al. Knowledge and practices related to bovine brucellosis transmission amongst livestock workers in Yewa, south-western Nigeria. J South Afr Vet Asso 2013;84(1):Art. #121, 5 pages.

Hambolu D, Freeman J, Taddese HB. Predictors of Bovine TB risk behaviour amongst meat handlers in Nigeria: a cross-sectional study guided by the Health Belief Model. PLoS ONE 2013;8(2):e56091.

Bloomfield SF, Nath KJ. Home Hygiene in Developing Countries: Prevention of infection in the home and the peri-domestic setting. Training resource for developing countries. International Scientific Forum on Hygiene 2013. Available at:


Adesokan HK, Raji AO. Safe meat-handling knowledge, attitudes and practices of private and government meat processing plants’ workers: implications for future policy. J Prev Med Hyg 2014;54:10-16.

Ali FHM, Mahdey EA. Incidence of Brucella species in slaughtered food animals and its edible offal at Beni-suef, Egypt. Global Vet 2010;5(5):248-254.

Ocholi RA, Kwaga JKP, Ajogi I. Phenotypic characterization of Brucella strains isolated from livestock in Nigeria. Vet Microbiol 2004;103:47–53.

Adamu NB. Epidemiology of Brucella infection in ruminants and humans and its public

health implications in Borno state, Nigeria. PhD thesis. Ahmadu Bello University, Zaria,

Nigeria: Veterinary Public Health and Preventive Medicine Department. 2009.

Courtenay WH. Constructions of masculinity and their influence on men’s well-being: a theory of gender and health. Social Science and Med 2000;50:1385–1401.

Davidson KW, Trudeau KJ, van Roosmalen E, et al. Perspective: gender as a health determinant and implications for health education. Hlth Educ Behaviour 2006;33:731–743.

European Commission. Risk issues. Special Eurobarometer 2006. Available at:

(Accessed September 04 2015). Nielsen K, Duncan B. Orlando, editors

McCarthy M, Brennan M, Kelly AL. et al. Who is at risk and what do they know? Segmenting a population on their food safety knowledge. Food Quality and Preference 2007;18:205-217.

Unusan N. Consumer food safety knowledge and practices in the home in Turkey. Food Control 2007;18(1):45-51.

Altekruse SF, Street DA, Fein SB, et al. Consumer knowledge of foodborne microbial hazards and food-handling practices. J Food Prot 1996;59:287-294.

Akhvlediani T, Clark DV, Chubabria G, et al. The changing pattern of human brucellosis: clinical manifestations, epidemiology, and treatment outcomes over three decades in Georgia. BMC Infect Dis 2010;10:346.

Kumar P, Singh DK, Barbuddhe SB. Sero-prevalence of brucellosis among abattoir personnel of Delhi. J Commun Dis 1997;29:131-137.

Ramos TRR, Junior JWP, Sobrinho PAM, et al. Epidemiological aspects of an infection by Brucella abortus in risk occupational groups in the microregion of Araguaina, Tocantins. Braz J Infect Dis 2008;12:133-138.

Mai H, Irons P, Thompson P. A large seroprevalence survey of brucellosis in cattle herds under diverse production systems in northern Nigeria. BMC Vet Res 2012;8:144.

Cadmus SIB, Alabi PI, Adesokan HK, et al. Serological investigation of bovine brucellosis in three cattle production systems in Yewa Division, south-western Nigeria. J South Afr Vet Asso 2013;84 (1):Art. #217, 6 pages.

Walshe MJ, Grindle J, Nell A, et al. Dairy development in sub- Saharan Africa, World Bank Technical Paper 135, Africa Technical Department Series, 1991; World Bank, Washington DC.

Alavi SM, Rafiei A, Nikkhooi A. The effect of lifestyle on brucellosis among nomads in Khuzestan Province of Iran. Pakistan J Med Sci 2007;23:358–360.