Sea buckthorn bud extract displays activity against cell-cultured Influenza virus

Alessandro Torelli, Elena Gianchecchi, Simona Piccirella, Alessandro Manenti, Giulia Piccini, Elisa Llorente Pastor, Brenno Canovi, Emanuele Montomoli

Abstract


Introduction - Vaccines and antiviral drugs are the most widely used methods of preventing or treating Influenza virus infection. The role of sea buckthorn (SBT) bud dry extract as a natural antiviral drug against Influenza was investigated.

Methods - Influenza virus was cultured in the MDCK cell line, with or without SBT bud extract, and virus growth was assessed by HA and TCID50 virus titration in terms of cytopathic effect on cells. Several concentrations of extract were tested, the virus titer being measured on day 4 after infection.

Results - After infection, the virus titer in the control sample was calculated to be 2.5 TCID50/ml; treatment with SBT bud extract reduced the virus titer to 2.0 TCID50/ml at 50 µg/ml, while the HA titer was reduced from 1431 (control) to 178. Concentrations lower than 50 µg/ml displayed an inhibitory effect in the HA assay, but not in the TCID50 virus titration; however, observation of the viral cultures confirmed a slowdown of viral growth at all concentrations.

Discussion - Natural dietary supplements and phytotherapy are a growing market and offer new opportunities for the treatment of several diseases and disorders. These preliminary experiments are the first to show that SBT bud extract is able to reduce the growth of the Influenza A H1N1 virus in vitro at a concentration of 50 µg/ml. This discovery opens up the possibility of using SBT bud extract as a valid weapon against Influenza and, in addition, as the starting-point for the discovery of new drugs.


Keywords


Sea buckthorn plant extract; Influenza prevention; antivirals

Full Text:

PDF

References


Loregian A, Mercorelli B, Nannetti G, Compagnin C, Palù G. Antiviral strategies against influenza virus: towards new therapeutic approaches. Cell Mol Life Sci 2014 Apr;71(19):3659-3683. Available from: https://www.nlm.nih.gov/medlineplus/flu.html PubMed PMID: 24699705. doi: 10.1007/s00018-014-1615-2. [Google Scholar]

Poovorawan Y, Pyungporn S, Prachayangprecha S, Makkoch J. Global alert to avian influenza virus infection: from H5N1 to H7N9.. Pathog Glob Health 2013;107(5):217-223. Available from: http://europepmc.org/abstract/MED/23916331 PubMed PMID: 23916331. doi: 10.1179/2047773213Y.0000000103. [Google Scholar]

Killip MJ, Fodor E, Randall RE. Influenza virus activation of the interferon system. Virus Res. 2015;09:10-1016. [Google Scholar]

Available from: http://www.who.int/mediacentre/factsheets/fs211/en/

Talbot HK, Griffin MR, Chen Q, Zhu Y, Williams JV, Edwards KM. Effectiveness of seasonal vaccine in preventing confirmed influenza-associated hospitalizations in community dwelling older adults.. J Infect Dis 2011 Jan;203(4):500-508. Available from: http://www.jid.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=21220776 PubMed PMID: 21220776. doi: 10.1093/infdis/jiq076. [Google Scholar]

Moscona A. Medical management of influenza infection.. Annu Rev Med 2008;59(1):397-413. Available from: http://www.scholaruniverse.com/ncbi-linkout?id=17939760 PubMed PMID: 17939760. doi: 10.1146/annurev.med.59.061506.213121. [Google Scholar]

Krug RM. Influenza: An RNA-synthesizing machine. Nature 2014 Dec;516(7531):338-339. Available from:

http://www.nature.com/doifinder/10.1038/516338a PubMed PMID: 25519129. doi: 10.1038/516338a. [Google Scholar]

Pinto LH, Lamb RA. The M2 proton channels of influenza A and B viruses. J Biol Chem 2006;281(14):8997-9000. Available from: http://www.jbc.org/cgi/doi/10.1074/jbc.R500020200 doi: 10.1074/jbc.R500020200. [Google Scholar]

Available from: http://www.ncbi.nlm.nih.gov/pubmed/23565231 PubMed PMID: 23565231. doi: 10.1371/journal.pone.0060348. [Google Scholar]

Deyde VM, Xu X, Bright RA, et al. Surveillance of resistance to adamantanes among influenza A(H3N2) and A(H1N1) viruses isolated worldwide. J Infect Dis 2007;196(2):249-257. Available from: http://jid.oxfordjournals.org/lookup/doi/10.1086/518936 doi: 10.1086/518936. [Google Scholar]

Bloom JD, Gong LI, Baltimore D. Permissive secondary mutations enable the evolution of influenza oseltamivir resistance.. Science 2010 Jun;328(5983):1272-1275. Available from: http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=20522774 PubMed PMID: 20522774. doi: 10.1126/science.1187816. [Google Scholar]

Gasparini R, Amicizia D, Lai PL, et al. Compounds with antiinfluenza activity: present and future of strategies for the optimal treatment and management of influenza. Part I: Influenza life-cycle and currently available drugs. J Prev Med Hyg 2014;55:69. [Google Scholar]

Gasparini R, Amicizia D, Lai PL, Bragazzi NL, Panatto D. Compounds with anti-influenza activity: present and future of strategies for the optimal treatment and management of influenza. Part II: Future compounds against influenza virus.. J Prev Med Hyg 2014;55(4):109-129. Available from: http://europepmc.org/abstract/MED/26137785 PubMed PMID: 26137785. [Google Scholar]

Shipulina LD, Tolkachev ON, Krepkova LV, et al. Seabuckthorn (Hippophae L.): A Multipurpose Wonder Plant. Vol. 2. New Delhi, India: Daya Publishing House; 2005. [Google Scholar]

Usha T, Middha SK, Goyal AK, Karthik M, Manoj D, Faizan S, et al. Molecular docking studies of anti-cancerous candidates in Hippophae rhamnoides and Hippophae salicifolia.. J Biomed Res 2014 May;28(5):406-415. Available from: http://dx.doi.org/10.7555/JBR.28.20130110 PubMed PMID: 25332713. doi: 10.7555/JBR.28.20130110. [Google Scholar]

Padwad Y, Ganju L, Jain M, Chanda S, Karan D, Kumar Banerjee P, et al. Effect of leaf extract of Seabuckthorn on lipopolysaccharide induced inflammatory response in murine macrophages. Int Immunopharmacol 2006;6(1):46-52. Available from: http://toxnet.nlm.nih.gov/cgi-bin/sis/search2/r?dbs+hsdb:@term+@rn+10102-43-9 PubMed PMID: 16332512. doi: 10.1016/j.intimp.2005.07.015. [Google Scholar]

Chauhan S, Varshneya C. The profile of bioactive compounds in Seabuckthorn: berries and seed oil. International Journal of Theoretical & Applied Sciences 2012;4:216. [Google Scholar]

Geetha S, Singh V, Ram MS, Ilavazhagan G, Banerjee PK, Sawhney RC. Immunomodulatory effects of seabuckthorn (Hippophae rhamnoides L.) against chromium (VI) induced immunosuppression. Mol Cell Biochem 2005;278(1-2):101-109. Available from: http://toxnet.nlm.nih.gov/cgi-bin/sis/search2/r?dbs+hsdb:@term+@rn+134-03-2 PubMed PMID: 16180095. doi: 10.1007/s11010-005-7095-9. [Google Scholar]

Ganju L, Padwad Y, Singh R, Karan D, Chanda S, Chopra MK, et al. Anti-inflammatory activity of Seabuckthorn (Hippophae rhamnoides) leaves. Int Immunopharmacol 2005;5(12):1675-1684. Available from: http://toxnet.nlm.nih.gov/cgi-bin/sis/search2/r?dbs+hsdb:@term+@rn+7440-26-8 PubMed PMID: 16102517. doi: 10.1016/j.intimp.2005.03.017. [Google Scholar]

Vermenichev SM. Proceedings of the 2nd All-Union Symposium on phenolic compounds held 17–21 May 1971 in Alma-Ata, 'Nauka' Kazakh, SSR (1973) Experimental study of toxicity and antitumor activity of natural and synthetic compounds of pyrone series. Phenolic compounds and their physiological properties; pp. 210–214.

DTs T, Rasputina DB, Zalykeeva DN, et al. A study of leaves and the oil cake of Seabuchthorn. Nauka Sibirdiv, Novosibirsk: Biology, Chemistry and Pharmacology of Seabuckthorn; 1983. [Google Scholar]

Negi PS, Chauhan AS, Sadia GA, et al. Antioxidant and antibacterial activities of various seabuckthorn (Hippophae rhamnoides L.) seed extracts. Food Chemistry 2005;92(1):119-124. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0308814604005643 doi: 10.1016/j.foodchem.2004.07.009. [Google Scholar]

Chauhan AS, Negi PS, Ramteke RS. Antioxidant and antibacterial activities of aqueous extract of Seabuckthorn (Hippophae rhamnoides) seeds. Fitoterapia 2007;78(7-8):590-592. Available from: https://www.nlm.nih.gov/medlineplus/antibiotics.html PubMed PMID: 17651916. doi: 10.1016/j.fitote.2007.06.004. [Google Scholar]

Jain M, Ganju L, Katiyal A, Padwad Y, Mishra KP, Chanda S, et al. Effect of Hippophae rhamnoides leaf extract against Dengue virus infection in human blood-derived macrophages. Phytomedicine 2008;15(10):793-799. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0944711308000925 doi: 10.1016/j.phymed.2008.04.017. [Google Scholar]

Xu MY, Sun XX, Tong WX. Medical research and development of Seabuckthorn. Hippophae 1994;7:32. [Google Scholar]

Beveridge T, Li TSC, Oomah, B. Dave, , Smith A. Sea Buckthorn Products: Manufacture and Composition †. J. Agric. Food Chem 1999;47(9):3480-3488. Available from: http://pubs.acs.org/doi/abs/10.1021/jf981331m doi: 10.1021/jf981331m. [Google Scholar]

Kocik J, Kołodziej M, Joniec J, Kwiatek M, Bartoszcze M. Antiviral activity of novel oseltamivir derivatives against some influenza virus strains.. Acta Biochim Pol 2014 Sep;61(3):509-513. Available from: http://www.actabp.pl/pdf/3_2014/509.pdf PubMed PMID: 25210935. [Google Scholar]

Committee on Infectious Diseases , author . Recommendations for Prevention and Control of Influenza in Children, 2012-2013. Pediatrics 2012;130(4):780-792. Available from: http://pediatrics.aappublications.org/cgi/doi/10.1542/peds.2012-2308 doi: 10.1542/peds.2012-2308. [Google Scholar]

Gaush CR, Hard WL, Smith TF. Characterization of an established line of canine kidney cells (MDCK).. Proc Soc Exp Biol Med

;122(3):931-935. Available from: https://www.lens.org/lens/search?q=citation_id:5918973 PubMed PMID: 5918973. [Google Scholar]

High yield production of influenza virus in Madin Darby canine kidney (MDCK) cells with stable knockdown of IRF7.. . Available from: http://www.ncbi.nlm.nih.gov/pubmed/23555825 PubMed PMID: 23555825. doi: 10.1371/journal.pone.0059892. [Google Scholar]

Hirst GK. The quantitative determination of influenza virus and antibodies by means of red cell agglutination. J Exp Med 1942;75:49. [Google Scholar]

Salk JE. A simplified procedure for titrating hemagglutinating capacity of influenza-virus and the corresponding antibody. J Immunol 1944;49:87. [Google Scholar]

Spearman C. The Method of 'Right and Wrong Cases' (Constant Stimuli) without Gauss' Formulae. Brit Jour of Psych. 1908;2

Lugovtsev VY, Melnyk D, Weir JP. Heterogeneity of the MDCK cell line and its applicability for influenza virus research.. PLoS One 2013 Sep;8(9). Available from: http://dx.plos.org/10.1371/journal.pone.0075014 PubMed PMID: 24058646. doi: 10.1371/journal.pone.0075014. [Google Scholar]




DOI: http://dx.doi.org/10.15167/2421-4248/jpmh2015.56.2.486

Refbacks

  • There are currently no refbacks.




©2018 - Journal of Preventive Medicine and Hygiene - jpmh@jpmh.org - ISSN 2421-4248